Journal
BIORESOURCE TECHNOLOGY
Volume 183, Issue -, Pages 120-128Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.02.027
Keywords
Anaerobic digestate; Cellic (R) CTec 2 saccharification; Ethanol fermentation; Feedlot cattle manure; Dilute-acid pretreatment
Funding
- NSW Environmental Trust [2012/RD/0047]
- NSW Department of Primary Industries, Australia
- Brazilian National Council for the Improvement of Higher Education
- CAPES [10225/12-9]
- Program of Research (FAP) from the University of Santa Cruz do Sul (UNISC) Brazil
- University of New South Wales, Australia
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This paper reports on processing options for the conversion of feedlot cattle manures into composite sugars for ethanol fermentation. Small-scale anaerobic digestion trials revealed that the process significantly reduces the content of glucan and xylan (ca. 70%) without effecting lignin. Moreover, anaerobic digestate (AD) fibres were poor substrates for cellulase (Cellic (R) CTec 2) saccharification, generating a maximum combined sugar yield of ca. 12% per original dry weight. Dilute acid pretreatment and enzyme saccharification of raw manures significantly improved total sugar recoveries, totalling 264 mg/g (79% theoretical). This was attained when manures were pretreated with 2.5% H2SO4 for 90 min at 121 degrees C and saccharified with 50 FPU CTec 2/g glucan. Saccharomyces cerevisiae efficiently fermented crude hydrolysates within 6 h, yielding 7.3 g/L ethanol, representing glucose to ethanol conversion rate of 70%. With further developments (i.e., fermentation of xylose), this process could deliver greater yields, reinforcing its potential as a biofuel feedstock. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.
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