Journal
CELLULOSE
Volume 27, Issue 3, Pages 1441-1450Publisher
SPRINGER
DOI: 10.1007/s10570-019-02890-3
Keywords
Filter paper; Xylan; Enzymatic hydrolysis; High solids loading; Hydrophilic lignin
Funding
- Program of Natural Science Foundation of Shaanxi Province [2018JQ5134]
- National Natural Science Foundation of China [51876206]
- Science and Technology Planning Project of Xi'an city [20193039YF027NS027]
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources [SKLCUSA-b201802]
- CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion [Y907k51001]
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Filter paper and xylan were selected as model substrates simulating cellulose and hemicellulose components. Enzymatic hydrolysis of different substrates: pure filter paper, xylan added system, alkali pretreated sugarcane bagasse (SCB) were studied at varying solids loading to assess the effect of hemicellulose on substrate conversion at high solids loading. Meanwhile, hydrophilic lignin (sulfonated lignin or alkali lignin) was added to 15% (w/v) alkali-pretreated SCB hydrolysis system to investigate lignin influence on cellulose digestibility. It was found that xylan as the physical barrier had inhibitory effect on cellulose hydrolysis. Enzymatic saccharification of alkali-pretreated SCB was significantly improved by supplementing with hydrophilic lignin. The effect of lignin on substrate conversion depended not only on the hydrophilic characteristics of lignin, but also on the form of lignin present in different substrates.
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