Journal
CELL
Volume 180, Issue 2, Pages 373-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2019.12.029
Keywords
-
Categories
Funding
- Yingwei Cui and Hui Zhao Neuroscience Fund
- Genentech Foundation predoctoral fellowship
- Vanessa Kong-Kerzner graduate fellowship
- Stanford Bio-X Bowes graduate fellowship
- National Institutes of Health [1K99-AG062746, U24-CA210979, U24-CA210986, U01-CA214125, R01-DK121409, R01-CA186568, R01-DC005982]
- Wu Tsai Neurosciences Institute of Stanford University (Neuro-omics grant)
Ask authors/readers for more resources
Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type- specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic analysis further revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map. These findings highlight the power of temporally resolved in situ cell-surface proteomic profiling in discovering regulators of brain wiring.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available