Journal
CELL
Volume 179, Issue 7, Pages 1647-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2019.11.025
Keywords
-
Categories
Funding
- Erling-Persson family foundation
- Knut and Alice Wallenberg Foundation
- Swedish Research Council
- Jonasson Center at the Royal Institute of Technology (Sweden)
- StratRegen at KI
- Knut and Wallenberg Foundation as part of the National Bioinformatics Infrastructure Sweden at SciLifeLab
- Formas [2017-01066_3]
- Swedish Research Council [2017-01066] Funding Source: Swedish Research Council
- Forte [2017-01066] Funding Source: Forte
Ask authors/readers for more resources
The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available