Novel quaternarized N-halamine chitosan and polyvinyl alcohol nanofibrous membranes as hemostatic materials with excellent antibacterial properties

The aim of this study was to develop novel nanofibrous membranes based on the quaternary ammonium N-halamine chitosan (CSENDMH) and polyvinyl alcohol (PVA) for antibacterial and hemostasis wound dressing. To improve the antimicrobial properties of nanofibrous membranes, a new chitosan-quaternary ammonium N-halamine derivative was successfully synthesized, and the structure was analyzed by 1H NMR and 13C NMR, fourier transform infrared (FTIR) spectroscopy, and elemental analysis. The morphological and water absorption ability studies showed that the membrane had a uniform bead-free network and high porosity structure like natural extracellular matrix as well as high hydrophilicity. For in vitro evaluation of the hemostatic effect, the membranes showed excellent blood clotting capacity, especially the PVA/CSENDMH membranes. The anti-microbial assay demonstrated excellent antibacterial activity of nanofibrous membranes against both gram-negative and gram-positive bacteria. Furthermore, the cytocompatibility assay results indicated that human fibroblasts could adhere and proliferate on the membranes, thus corroborating their biocompatibility.