Journal
CARBOHYDRATE POLYMERS
Volume 229, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115521
Keywords
beta-Glucans; alpha-Glucans; Shiitake mushroom; Hypocholesterolemic; Anti-inflammatory; Cytotoxic
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Funding
- Spanish Ministry of Science and Innovation [AGL2014-56211-R]
- Community of Madrid, Spain [S2013/ABI-2728]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
- Fundacao Araucaria
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A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with beta-(1 -> 6), beta-(1 -> 3),(1 -> 6) and alpha-(1 -> 3) linkages, being characterized through GC-MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory and antioxidant activities were also tested. Isolated glucans exerted HMGCR inhibitory activity, but only beta-(1 -> 6) and beta-(1 -> 3),(1 -> 6) fractions showed DPPH scavenging capacity. Glucans were also able to lower IL-1 beta and IL-6 secretion by LPS-activated THP-1/M cells and showed cytotoxic effect on a breast cancer cell line that was not observed on normal breast cells. These in vitro results pointed important directions for further in vivo studies, showing different effects of each chemical structure of the isolated glucans from shiitake mushrooms.
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