Journal
CANCER SCIENCE
Volume 111, Issue 5, Pages 1582-1595Publisher
WILEY
DOI: 10.1111/cas.14372
Keywords
EZH2; hepatocellular carcinoma; MALAT1; miR-22; SNAI1
Categories
Funding
- Natural Science Foundation of Hubei Province [2019CFB496]
- Huazhong University of Science and Technology Innovation Research Fund [2017KFYXJJ251]
- National Key Basic Research Program of China [2015CB5540007]
- National Natural Science Foundation of China [81772967]
- Funds for training young and middle-aged medical backbone talents in Wuhan [2017KFYXJJ251]
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Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is an oncogenic long noncoding RNA that has been found to promote carcinogenesis and metastasis in many tumors. However, the underlying role of MALAT1 in the progression and metastasis of hepatocellular carcinoma (HCC) remains unclear. In this study, aberrantly elevated levels of MALAT1 were detected in both HCC specimens and cell lines. We found that knockdown of MALAT1 caused retardation in proliferation, migration, and invasion both in vivo and in vitro. Mechanistic investigations showed that Snail family transcriptional repressor 1 (SNAI1) is a direct target of microRNA (miR)-22 and that MALAT1 modulates SNAI1 expression by acting as a competing endogenous RNA for miR-22. Inhibition of miR-22 restored SNAI1 expression suppressed by MALAT1 knockdown. Furthermore, MALAT1 facilitated the enrichment of enhancer of zeste homolog 2 (EZH2) at the promoter region of miR-22 and E-cadherin, which was repressed by MALAT1 knockdown. Cooperating with EZH2, MALAT1 positively regulated SNAI1 by repressing miR-22 and inhibiting E-cadherin expression, playing a vital role in epithelial to mesenchymal transition. In conclusion, our results reveal a mechanism by which MALAT1 promotes HCC progression and provides a potential target for HCC therapy.
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