Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 66, Issue 4, Pages 328-336Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/cjm-2019-0554
Keywords
STEC; biofilm formation; stainless steel; temperature
Categories
Funding
- National Research Foundation of South Africa [98983]
- North-West University
- Growing Forward II (GF2) of Alberta Agriculture and Forestry, the Agriculture and Agri-Food Canada-Beef Cluster program
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2019-04384]
- Canada Foundation for Innovation
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This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 degrees C and after 168 h at 10 degrees C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on both the submerged surface and the air-liquid interface of coupons, whereas strains O116:H21, wzx-Onovel5:H19, O129:H21, and O26:H11 formed biofilm only at the air-liquid interface. Viable cell counts and scanning electron microscopy showed that biofilm formation increased (p < 0.05) over time. The biofilm-forming ability of non-O157 STEC was strongest (p < 0.05) at 22 degrees C after 48 h of incubation. The strongest biofilm former regardless of temperature was O129:H23. Generally, at 10 degrees C, weak to no biofilm was observed for isolates O154:H10, O116:H21, wzx-Onovel5:H19, O26:H11, and O129:H21 after 168 h. This study found that temperature affected the biofilm-forming ability of non-O157 STEC strains. Overall, our data indicate a high potential for biofilm formation by the isolates at 22 degrees C, suggesting that non-O157 STEC strains could colonize stainless steel within food-processing facilities. This could serve as a potential source of adulteration and promote the dissemination of these potential pathogens in food.
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