Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 189, Issue 4, Pages 760-771Publisher
WILEY
DOI: 10.1111/bjh.16439
Keywords
platelet function; immune thrombocytopenia; platelet antibodies; flow cytometry
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology in Japan
- Ministry of Health, Labor and Welfare in Japan
- SENSHIN Medical Research Foundation
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Platelet function of immune thrombocytopenia (ITP) has been controversial because of methodological problems associated with low platelet counts. In this study, we evaluated platelet function in 21 patients with chronic ITP (cITP) using the recently developed flow cytometry (FCM)-based platelet aggregation assay (FCA) along with a PAC1/CD62P assay. Since ITP platelets are larger than controls, whole platelets (whole gating method) and size-adjusted platelets (size-adjusted method) were analysed in the PAC1/CD62P via FCM. We found that: (i) aggregation was equivalent [phorbol myristate acetate (PMA) or adenosine diphosphate (ADP)-induced] or enhanced [protease-activated receptor 1-activating peptide (PAR1AP)-induced] in cITP compared with control by FCA; (ii) PAC1 or CD62P was also equivalent or enhanced in cITP in the whole gating method; and (iii) in sharp contrast, the size-adjusted method revealed that ADP-, PAR1AP-, and collagen synthetic liquid reactive peptide (SRP)-induced PAC1 and ADP-induced CD62P were impaired in cITP. These data suggested that an increase in the number of larger-sized platelets may compensate for the impaired platelet function of cITP, leading to non-inferiority of overall platelet function in cITP. Furthermore, we revealed that ADP-induced aggregation was impaired in the patients with thrombopoietin receptor agonists (TPO-RAs) or platelet-associated anti-alpha IIb beta 3 antibodies compared with the control, suggesting that the presence of anti-alpha IIb beta 3 autoantibodies and/or administration of TPO-RAs may have a negative impact on platelet function.
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