Journal
BMC PLANT BIOLOGY
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12870-020-2320-y
Keywords
Brassinolide; Nitric oxide; Nitric oxide synthase; Nitrate reductase; Nitrate reductase genes
Categories
Funding
- National Natural Science Foundation of China [31660584]
- Agriculture Research System of China [CARS-23-C-07]
- Gansu Province Science and Technology Project [17ZD2NA015-03]
- National Key Research and Development Project of China [2018YFD0201205]
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Background Brassinolide (BR), as a new type of plant hormones, is involved in the processes of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. The main purpose of our study was to explore whether nitric oxide (NO) plays a role in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.). Results Exogenous application of 1 mu M BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 mu M) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP applied together significantly promoted adventitious rooting and the combined effect was superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 mu M also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion BR promoted the formation of adventitious roots by inducing the production of endogenous NO in cucumber.
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