Journal
BIOSENSORS & BIOELECTRONICS
Volume 152, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112027
Keywords
chemiluminescence; Nanozyme; Phosphatase; Biocatalyst; Enzyme mimic
Categories
Funding
- National Natural Science Foundation of China (NSFC) [21605012, 21705012]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900522]
- Venture & Innovation Support Program for Chongqing Overseas Returnees
Ask authors/readers for more resources
Nanomaterials with enzyme-like characteristics (also called nanozymes) have attracted increasing attention in the area of analytical chemistry. Nevertheless, most of the nanozymes used for analytical applications are oxidoreductase mimics, and their enzyme-like activities are usually demonstrated by using chromogenic and/or fluorogenic substrates. Herein, the phosphatase mimetic activity of cerium oxide nanoparticles (nanoceria) was investigated by using CDP-star as the chemiluminescent (CL) substrate. Interestingly, we found that the phosphatase mimetic activity of nanoceria can be remarkably inhibited by the addition of Al3+ . Based on this finding, a highly sensitive and selective CL method for Al3+ detection is proposed. The CL intensity of the nanoceria/CDP-star system decreased with the increasing Al3+ concentrations in the range from 30 nM to 3.5 mu M. A detection limit as low as 10 mu M was obtained. Finally, the CL detection of intracellular Al3+ was achieved, demonstrating the utility of the CL method in complex biological samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available