Journal
BIORESOURCE TECHNOLOGY
Volume 189, Issue -, Pages 162-168Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.04.011
Keywords
Extraction; Microalgae; CO2-expanded ethanol; Lipid; Yield
Funding
- ROC Ministry of Science and Technology [NSC103-3113-P-007-004]
- National Tsing Hua University at Hsinchu, Taiwan, ROC
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CO2-expanded ethanol (CXE) was used to extract DHA-containing lipids from Schizochytrium sp. with a 35.7 wt% lipid content of dry biomass in a continuous mode. The effects of operation variables such as temperature, pressure, ethanol flow rate and CO2 flow rate on extraction performance were investigated. Based on a 24-central composite design and response surface methodology, the optimal operating conditions were determined to be a pressure of 6.9 MPa, a temperature of 313 K, an ethanol flow rate of 1 mL/min and a CO2 flow rate of 6.0 mL/min, providing an extracted lipid yield of 87 wt% over an extraction period of 30 min. Not only the lipid yield obtained using CXE was observed to be significantly greater than those using ethanol and pressurized ethanol as the solvents, but also a lower amount of ethanol and less time were required to achieve the same extraction yield by using CXE. (C) 2015 Elsevier Ltd. All rights reserved.
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