Journal
BIOSENSORS & BIOELECTRONICS
Volume 145, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111719
Keywords
Prostate cancer; Free and complexed prostate-specific antigen; Magnetic-quantum dot nanobead; Fluorescent lateral flow immunoassay; Simultaneous detection; Point-of-care
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Funding
- National Natural Science Foundation of China [81902159]
- National S&T Major Project for Infectious Diseases Control [2018ZX10712001-010, 2018ZX10101003-001]
- Natural Science Foundation of Anhui Province [1908085QB85]
- Natural Foundation of Jiangsu Province [BK20140735]
- Beijing National Day School
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Simultaneous detection of free and complexed prostate-specific antigen (f-PSA and c-PSA) is critical to the prostate cancer (PCa) diagnostic accuracy for clinical samples with PSA values in the diagnostic gray zone between 4 and 10 ng mL(-1). Herein, red and green magnetic-quantum dot nanobeads (MQBs) with superior magnetic property and high luminescence were fabricated via polyethyleneimine-mediated electrostatic adsorption of numerous quantum dots onto superparamagnetic Fe(3)O(4 )magnetic cores, and were conjugated with f-PSA antibody and c-PSA antibody, respectively, as versatile fluorescent probes in test strip for immune recognition, magnetic enrichment, and simultaneous detection of f-PSA and c-PSA analytes in complex biological matrix with t-PSA antibody on the test line. A low-cost and portable smartphone readout device with an application was also developed for the imaging of dual-color test strips and data processing. This assay can simultaneously detect f-PSA and c-PSA with the limits of detection of 0.009 ng mL(-1) and 0.087 ng mL(-1), respectively. Clinical serum samples of PCa and benign prostatic hyperplasia patients were evaluated to confirm the clinical feasibility. The results suggest that the proposed dual-color MQBs-based fluorescent lateral flow immunoassay is a promising point-of-care diagnostics technique for the accurate diagnosis of PCa even in resource-limited settings.
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