Journal
BIOSENSORS & BIOELECTRONICS
Volume 147, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111790
Keywords
Titanium dioxide; SnS2 quantum dots; Coreaction accelerator; Chloramphenicol; Electrochemiluminescence immunosensor
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Funding
- Science Fund from the National Natural Science Foundation of China [21175097, 31772053]
- Project of Scientific and Technologic Infrastructure of Suzhou [SZS201207]
- Project of State Key Labora-tory of Analytical Chemistry for Life Science [SKLACLS1716]
- Suzhou Industry, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials [SYG201636]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX10900212]
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In this study, modified hollow titanium dioxide and SnS2 quantum dots (SnS2 QDs) were used to build a novel electrochemiluminescence immunoassay (ECLIA) for ultrasensitive detection of chloramphenicol (CAP). The titanium dioxide hollow spheres (THS) modified with polyethyleneimine (PEI) and gold nanoparticles (Au NPs) was synthesized as a probe material for the novel ECLIA, meanwhile it also could be a coreaction accelerator which could greatly improve the interaction efficiency between SnS2 QDs and S2O82-. Then the modified THS was combined with chloramphenicol antibody to form ECLIA probes, and colloidal gold synthesized as a platform for an ECLIA by ascorbic acid (AA) reduction. A competitive immunoassay strategy was used for the detection of CAP, where CAP in the sample would compete with the coating antigen for the limited antibodies. The proposed ECLIA for CAP detection exhibited high sensitivity with a wide linear range from 0.01 ng mL(-1) to 100 ng mL(-1) and a low detection limit at 3.1 pg mL(-1). Furthermore, it could be seen from electrochemical analysis that the effect of THS on the enhancement of ECL signal is about twice as high as that of ordinary titanium dioxide. Importantly, this work not only successfully applied THS to amplify electrochemical-luminescence signal in the ECLIA, but also successfully prepared a stable and highly selective sensor for ultrasensitive detection of CAP.
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