Journal
BIOSENSORS & BIOELECTRONICS
Volume 145, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111624
Keywords
E. coli detection; Smartphone readout; Point-of-care diagnostics; Synthetic urine; UTIs; Microcapillary Film
Categories
Funding
- Loughborough University
- Santander Universities for the Mobility Award [C10973/2055]
- EPSRC project [EP/M027341/1]
- EPSRC [EP/M027341/1] Funding Source: UKRI
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In spite of the clinical need, there is a major gap in rapid diagnostics for identification and quantitation of E. coli and other pathogens, also regarded as the biggest bottleneck in the fight against the spread of antimicrobial resistant bacterial strains. This study reports for the first time an optical, smartphone-based microfluidic fluorescence sandwich immunoassay capable of quantifying E. colt in buffer and synthetic urine in less than 25 min without sample preparation nor concentration. A limit of detection (LoD) up to 240 CFU/mL, co-mensurate with cut-off for UTIs (10(3)-10(5) CFUs/mL) was achieved. Replicas of full response curves performed with 10(3)-10(0) CFUs/mL of E. colt K12 in synthetic urine yielded recovery values in the range 80-120%, assay reproducibility below 30% and precision below 20%, therefore similar to high-performance automated immunoassays. The unrivalled LoD was mainly linked to the 'open fluidics' nature of the 10-bore microfluidic strips used that enabled passing a large volume of sample through the microcapillaries coated with capture antibody. The new smartphone based test has the potential of being as a rapid, point-of-care test for rule-in of E. coli infections that are responsible for around 80% of UT15, helping to stop the over-prescription of antibiotics and the monitoring of patients with other symptomatic communicable diseases caused by E. coli at global scale.
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