Journal
BIOSENSORS & BIOELECTRONICS
Volume 145, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111638
Keywords
One-pot aqueous method; Electrochemical immunosensor; Tripods; Hierarchical branches; Cardiac troponin I
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Funding
- National Natural Science Foundation of China [21475118, 21675093, 21305124]
- Zhejiang Public Welfare Technology Application Research Project [LGG18E010001, LGG19B050001]
- Natural Science Foundation of Zhejiang Province [Q19B050016]
- Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology [SATM201703, STAM201804]
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By integrating the amplified electrochemical signals and effective capture of antibodies together, advanced multimetallic superstructured nanocrystals endow label-free immunosensors promising applications in early diagnosis and monitoring of diseases. Herein, four-metallic PtCoCuPd hierarchical branch-like tripods (HBTPs) were directly synthesized by a green one-pot aqueous method without any seed or organic solvent involved, which were applied to construct a novel label-free immunosensor for detecting cardiac troponin I (cTnI). The specific hierarchical micro/nanostructures greatly improved the immobilization of antibodies and enhanced the catalytic activity for K3Fe(CN)(6), which would effectively amplify the electrochemical signals, thereby improving the detection sensitivity. Under the optimal conditions, the as-developed immunosensor exhibited a wide linear range (0.001-100.0 ng mL(-1)) and a low detection limit (0.2 pg mL(-1), S/N = 3) for the assay of cTnl. The im- munosensor provides a powerful platform for quantitative detection of cTnI, which can be explored to detect other tumor markers in actual sample analysis.
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