Journal
BIORESOURCE TECHNOLOGY
Volume 293, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122169
Keywords
Denitrification; Ammonia cycle; Metabolism; Pseudomonas sp. C27
Funding
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2018137]
- Scientific Research Project of Harbin University of Commerce [18XN044]
- Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology [HC201710]
- Harbin Applied Technology Research and Development Project [2017RAXXJ025]
Ask authors/readers for more resources
Pseudomonas sp. C27 is a facultative autotrophic bacterium that can grow mixotrophically to undergo denitrifying sulfide removal (DSR) reactions with both organic matters and sulfide as electron donors. A detailed understanding of how the C27 strain simultaneously removes nitrogen, sulfur and carbon from water is critical for optimal DSR process design and implementation. This study is the first to reveal the pathways of nitrogen and sulfur metabolisms, identifying a total of 47 proteins that are related to the nitrogen metabolism and seven proteins to the sulfur metabolism of strain C27 using iTRAQ and LC-MS/MS techniques. The proposed pathway of nitrogen metabolism for strain C27 from external nitrate to nitrogen gas and phosphate with a coupled ammonia cycle is based on the identified proteins, and suggests that nitrate was not essential for nitrogen metabolism and could be replaced by nitrite as the sole nitrogen source for C27.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available