Journal
BIORESOURCE TECHNOLOGY
Volume 295, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122191
Keywords
Anaerobic digestion; Ammonia stress; Metagenomics; Antibiotic resistance genes; Microbial community
Funding
- National Major Science & Technology Projects for Water Pollution Control and Management of China [2017ZX07102-002]
- National Natural Science Foundation of China [51808540]
- Natural Science Foundation of Beijing Municipality [8182053]
Ask authors/readers for more resources
The dynamics of antibiotic resistance genes (ARGs) response to ammonia stress were evaluated using metagenomics and quantitative PCR (qPCR) in anaerobic digestion (AD). Ammonia stress reduced ARGs associated with antibiotic efflux, especially the major facilitator superfamily (MFS) of tet(L), due to free ammonia (FA) that changed the proton gradient of efflux system. Nonetheless, ARGs of antibiotic target alteration, especially ermB, were enriched under ammonia stress, which could be attributed to the initiation of the internal enhancer of the transferability of the broad host range plasmid, pAMbeta1. Statistical analysis elucidated the significant changes of ARGs are directly attributed to the mobile genetic elements (MGEs), but the little affected ARGs are mainly determined by the functional microbes reflected by nitrogen cycling genes (NCyc). This study deciphered the profiles of ARGs response to ammonia stress in AD, which indicated the importance of alleviation of ammonia inhibition for the mitigation of ARGs dissemination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available