Journal
BIOMEDICINE & PHARMACOTHERAPY
Volume 122, Issue -, Pages -Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2019.109763
Keywords
Cinobufagin; Nasopharyngeal carcinoma; HK-1 cells; Mitochondria-mediated apoptosis; Cell cycle arrest
Funding
- National Natural Science Foundation of China [81872162, 81602556, 31870338]
- Shandong Provincial Natural Science Foundation, China [ZR2017JL030]
- Key Research and Development Program of Shandong Province of China [2019GSF108214]
- Taishan Scholars Construction Engineering of Shandong Province
- Yantai High-End Talent Introduction Plan Double Hundred
- Dominant Disciplines' Talent Team Development Scheme of Higher Education of Shandong Province
Ask authors/readers for more resources
Emerging evidence suggests that cinobufagin, an active ingredient in Venenum Bufonis, inhibits cell proliferation in several tumor cells. However, the anti-tumor effect of cinobufagin on nasopharyngeal carcinoma and the underlying molecular mechanisms are still unclear. In this study, we found that cinobufagin significantly inhibits the proliferation of nasopharyngeal carcinoma HK-1 cells. Further analyses demonstrated that cinobufagin induces cell cycle arrest at the S phase in HK-1 cells through downregulating the levels of CDK2 and cyclin E. Moreover, cinobufagin significantly downregulates the protein level of Bcl-2 and upregulates the levels of Bax, subsequently increasing the levels of cytoplasmic cytochrome c, Apaf-1, cleaved PARP1, cleaved caspase-3, and cleaved caspase-9, leading to HK-1 apoptosis. Furthermore, we found that cinobufagin significantly increases ROS levels and decreases the mitochondrial membrane potential in HK-1 cells. Collectively, these data imply that cinobufagin induces cell cycle arrest at the S phase and induces apoptosis through increasing ROS levels, thereby inhibiting cell proliferation in HK-1 cells. Therefore, cinobufagin is a promising bioactive agent that may contribute to the development of treatment strategies of nasopharyngeal carcinoma.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available