Journal
BIOCHEMISTRY
Volume 59, Issue 6, Pages 733-735Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b01101
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Funding
- National Science Foundation [MCB-1410854]
- National Cancer Institute [P30 CA016086]
- National Institutes of Health [T32 GM008570]
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We assessed the ability of two strains of Escherichia coli, BL21 (DE3) and Tuner (DE3), to express a variant of the B1 domain of protein G, which forms a side-by-side dimer, by using fluorine-labeling and F-19 nuclear magnetic resonance spectroscopy. BL21 cells express the protein in a binary, all-or-none, manner, where more cells express the protein at a high level with an increasing inducer concentration. Tuner cells express the protein in a rheostatic manner, where expression increases across all cells with an increasing inducer concentration.
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