4.6 Article

Examination of indigenous microalgal species for maximal protein synthesis

Journal

BIOCHEMICAL ENGINEERING JOURNAL
Volume 154, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.bej.2019.107425

Keywords

Chlorella; CO2 concentration; Light intensity; Microalgal protein; Nitrate concentration

Funding

  1. Fundamental Research Grant Scheme, Malaysia [FRGS/1/2015/SG05/UNIM/03/1]
  2. Ministry of Science, Technology and Innovation, Malaysia [MOSTI02-02-12-SF0256]
  3. Prototype Research Grant Scheme, Malaysia [PRGS/2/2015/SG05/UNIM/03/1]
  4. Public-Private Research Network (PPRN) fund

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The expanding aquaculture industry increases the prices of fishmeal, the main protein source in fish diet. A promising alternative is microalgal protein. Therefore, we investigated the protein production capacities of green microalgae Chlorella sorokiniana CY1 and Chlorella vulgaris ESP-31. After optimization, the maximum biomass and protein productivities of Chlorella sorokiniana CY1 reached high values of 4.35 +/- 0.09 and 0.856 +/- 0.025 g/L/d, while that of Chlorella vulgaris ESP-31 also reached high values of 4.636 +/- 0.10 and 0.946 +/- 0.065 g/L/d. The cultivation time for both species was only 2 days, wherein Chlorella sorokiniana CY1 and Chlorella vulgaris ESP-31 amassed moderate protein contents of 25.9 +/- 1.3% and 26.8 +/- 1.3%. The optimum conditions for both species were 50% initial nitrate concentration of Basal medium, 5% CO2 aeration, and 750 mu mol/m(2)/s light intensity. The high biomass and protein productivities of both species indicated their capability as potential protein sources.

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