Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 153, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bej.2019.107402
Keywords
Heterologous expression; Manganese peroxidase; Pichia pastoris; Dye decolorization; Denim bleaching; Polycyclic aromatic hydrocarbon bioremediation
Funding
- Ministry of Education, Singapore [MOE2013-TIF-1-G-062]
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Manganese peroxidase (MnP), which is an extracellular glycosylated heme protein produced by various basi-diomycetous fungi, shows attractive potential in various biotechnological applications. A novel MnP gene, mnp3, from white-rot fungus Cerrena unicolor BBP6 was cloned and expressed in Pichia pastoris with a MnP activity of 154.5 U/l. The recombinant enzyme, rMnP3-BBP6, had strong decolorizing activity on a variety of dyes and it was efficient in denim bleaching. In addition, it was able to degrade fluorene and phenanthrene effectively. The results of this research demonstrated that rMnP3-BBP6 had promising biotechnological application potential in textile industries and polycyclic aromatic hydrocarbon bioremediation.
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