Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 524, Issue 4, Pages 791-797Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2020.01.046
Keywords
Cell proliferation; MicroRNA; Polycystic ovary syndrome; Granulosa cell
Categories
Funding
- National Natural Science Foundation of China, China [81601238, 81671412]
- National Key Research and Development Program of China, China [2017YFC1001303]
- International Cooperation Project of China [81661128010]
- Canada NSFC China [81661128010]
- Chenguang Program by Shanghai Education Development Foundation [18CG08]
- Shanghai Municipal Education Commission, China [18CG08]
- Shanghai Municipal Commission of Health and Family Planning, China [20164Y0251]
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Increased granulosa cell (GC) proliferation may contribute to abnormal folliculogenesis in patients with polycystic ovary syndrome (PCOS), which affects approximately 10% reproductive aged women. However, the mechanisms underlying increased GC proliferation in PCOS remain incompletely understood. In this study, we identified miR-3940-5p as a hub miRNA in GC from PCOS using weighted gene co-expression network analysis (WGCNA), and real-time polymerase chain reaction (RT-PCR) analysis confirmed that miR-3940-5p was significantly increased in GC from PCOS. Enrichment analysis of predicted target genes of miR-3940-5p indicated potential roles of miR-3940-5p in follicular development and cell proliferation regulation. Consistently, functional study confirmed that miR-3940-5p overexpression promoted granulosa cell proliferation. Integrated analysis of mRNA expression profiling data and predicted target genes of miR-3940-5p identified potassium voltage-gated channel subfamily A member 5 (KCNA5) as a potential target of miR-3940-5p, and was validated by luciferase reporter assay. Finally, functional analysis suggested that miR-3940-5p promoted GC proliferation in a KCNA5 dependent manner. In conclusion, miR-3940-5p was a hub miRNA upregulated in GC from PCOS, and promoted GC proliferation by targeting KCNA5. (C) 2020 Elsevier Inc. All rights reserved.
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