Aggressive behavior and brain neuronal activation in sexually naive male Mongolian gerbils

Aggressive behavior plays an important role in animal's survival and reproductive success. Although there has been growing interests in studying neural mechanisms underlying aggressive behavior using traditional laboratory animal models, little is known about mechanisms controlling naturally occurring aggression in sexually naive animals. In the present study, we characterized aggressive behavior displayed by sexually naive male Mongolian gerbils (Meriones unguiculatus) and examined the subsequent neuronal activation in the brain measured by Fos-immunoreactive (Fos-ir) staining. We found that resident males initiated attacks and showed intense levels of aggression (including chase, bite, offensive sideway, lunge and on-top) towards a conspecific male intruder. Furthermore, attacks from the resident males towards the intruder produced a nonrandom distribution of bites, with the most on the rump, flank, back and tail and few on the limbs, ventrum and head. In contrast, control males that were exposed to a woodblock (control for novelty) never attacked the woodblock and showed higher levels of object/environmental investigation. Male gerbils exposed to an intruder had significantly higher levels of Fos-ir density in the medial (MeA) and anterior cortical (ACo) subnuclei of the amygdala, principal nucleus (BSTpr) and interfascicular nucleus (BSTif) of the bed nucleus of the stria terminalis, ventrolateral subdivision of the ventromedial hypothalamus (VMHvl), and paraventricular nucleus of the hypothalamus (PVN), compared to control males. Together, our results indicate that sexually naive, group housed male gerbils naturally display aggression towards conspecific strangers, and such aggressive behavior is associated with special patterns of neuronal activation in the brain.