Journal
ANTICANCER RESEARCH
Volume 39, Issue 12, Pages 6701-6709Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.13885
Keywords
Extracellular vesicles; exosomes; lyophilization; cell-penetrating peptides; saporin
Categories
Funding
- JSPS KAKENHI [JP16H02612, JP19H05553]
- JST CREST [JPMJCRI7H3]
- Leading University as a Base for Human Resource Development in Nanoscience and Nanotechnology, Osaka Prefecture University
Ask authors/readers for more resources
Background/Aim: Extracellular vesicles (exosomes, EVs) (30-200 nm in diameter) are secreted by various cells in the body. Owing to the pharmaceutical advantages of EVs, an EV-based drug delivery system (DDS) for cancer therapy is expected to be the next-generation therapeutic system. However, preservation methods for functional and therapeutic EVs should be developed. Here, we developed the method of lyophilization of arginine-rich cell penetrating peptide (CPP)-modified EVs and investigated the effects of lyophilization on the characteristics of EVs. Materials and Methods: Particle size, structure, zeta-potential, and cellular uptake efficacy of the arginine-rich CPP-modified EVs were analyzed. The model protein saporin (SAP), having anti-cancer effects, was encapsulated inside the EVs to assess the cytosolic release of EV content after cellular uptake. Results: Lyophilization of the EVs did not affect their particle size, structure, zeta-potential, and cellular uptake efficacy; however, the biological activity of the encapsulated SAP was inhibited by lyophilization. Conclusion: Lyophilization of EVs may affect SAP structures and/or reduce the cytosolic release efficacy of EV's content after cellular uptake and needs attention in EV-based DDSs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available