Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 11, Pages 4415-4420Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201911859
Keywords
bladder cancer; fluorescence imaging; imaging agents; renal clearance; urinalysis
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Funding
- Nanyang Technological University [M4081627]
- Singapore Ministry of Education Academic Research Fund Tier1 [2017-T1-002-134, RG147/17, 2019-T1-002-045, RG125/19]
- Academic Research Fund Tier2 [MOE2018-T2-2-042]
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Bladder cancer (BC) is a prevalent disease with high morbidity and mortality; however, in vivo optical imaging of BC remains challenging because of the lack of cancer-specific optical agents with high renal clearance. Herein, a macromolecular reporter (CyP1) was synthesized for real-time near-infrared fluorescence (NIRF) imaging and urinalysis of BC in living mice. Because of the high renal clearance (ca. 94 % of the injection dosage at 24 h post-injection) and its cancer biomarker (APN=aminopeptidase N) specificity, CyP1 can be efficiently transported to the bladder and specially turn on its NIRF signal to report the detection of BC in living mice. Moreover, CyP1 can be used for optical urinalysis, permitting the ex vivo tracking of tumor progression for therapeutic evaluation and easy translation of CyP2 as an in vitro diagnostic assay. This study not only provides new opportunities for non-invasive diagnosis of BC, but also reveals useful guidelines for the development of molecular reporters for the detection of bladder diseases.
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