Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 5, Pages 3795-3803Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05206
Keywords
-
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [371350234]
Ask authors/readers for more resources
In this work, we introduce a new two-dimensional chip-based high-performance liquid chromatography (2D chip-HPLC) approach, which enables multiple transfers from the first dimension effluent onto the column head of the second separation dimension. By merging injection, separation, and detection features on a fused silica chip in a dead volume-free manner, all extra-column peak dispersion effects can be reduced to an absolute minimum. The application of intrinsic fluorescence detection with excitation in the deep-UV spectral region and electrospray ionization mass spectrometry after the first and second separation dimension, respectively, enables the label-free analysis of complex samples, as exemplarily shown for a pesticide mixture and a tryptic digest.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available