Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 7, Pages 5091-5099Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05468
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [11727810, 21974050]
- Natural Science Foundation of Shanghai [18ZR1411300]
- Fundamental Research Funds for the Central Universities
Ask authors/readers for more resources
Myeloperoxidase (MPO) is a heme peroxidases protein associated with many inflammation-related diseases. Although many fluorescent probes have been constructed for the assessment of MPO activity, it still remains a challege to develop a nanoprobe for highly sensitive biosensing and high-resolution bioimaging in biological system. In this work, we developed a novel luminescent nanoprobe based on upconversion nanoparticles (UCNPs) conjugated with phycocyanin (PC), which could detect the fluctuation of MPO. By grafting PC onto the surface of UCNPs through amidation reaction, the luminescence of UCNPs is quenched by PC via energy transfer. Due to the specific recognition by PC, the nanoprobe can be used for sensitive evaluation the bioactivity of MPO. The nanoprobe based on PC-UCNPs has been successfully applied for the bioimaging of MPO in living cells and an inflammatory process by taking an acute liver injury mouse as a model.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available