Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 3, Pages 2809-2814Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05218
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Funding
- National Natural Science Foundation of China [21675029, 21874022]
- Health Education Joint Research Project of Fujian Province [WKJ2016-2-15]
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A self-powered temperature sensor based on Seebeck effect transduction was designed for photothermal-thermoelectric coupled immunoassay of alpha-fetoprotein (AFP). In this system, glucose oxidase (GOx)-conjugated detection antibody was first captured onto the microplate by target-induced sandwich-type immunoreaction. Thereafter, the as-generated hydrogen peroxide via the GOx-glucose system oxidized 3,3',S,S'-tetrametylbenzidine (TMB) into photothermal product oxidized TMB (ox-TMB). Under near-infrared (NIR) laser irradiation, the temperature change of ox-TMB was read out in an electrical signal by the flexible thermoelectric module in a 3D-printed integrated detection device. Under optimal conditions, the photothermal-thermoelectric coupled immunoassay exhibited a limit of detection of 0.39 ng mL(-1) AFP over a dynamic linear range from 0.5 to 60 ng mL(-1). Impressively, such a strategy presented herein offers tremendous potentials for applying many other high-efficiency thermoelectric materials in ultrasensitive biosensors.
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