Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 1, Pages 1122-1129Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04188
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Funding
- Ministere de l'Enseignement Superieur, de la Recherche et de l'Innovation (MESRI)
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- Universite de Lille
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Large scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size which confers them peptide-like physicochemical properties, they are more difficult to detect using standard proteomics strategies. In this study, we tested different preparation workflows for improving the identification of AltProts in NCH82 human glioma cell line. The highest number of identified AltProts was achieved with RIPA buffer or boiling water extraction followed by acetic acid precipitation.
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