Journal
ANALYTICAL BIOCHEMISTRY
Volume 593, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2020.113596
Keywords
Padlock probe; Rolling circle amplification; Microbeads; Molecular crowding; Poly(ethylene glycol); Excluded volume
Funding
- Foundation Advanced Technology Institute
- JSPS, KAKENHI [15K15199]
- Grants-in-Aid for Scientific Research [15K15199] Funding Source: KAKEN
Ask authors/readers for more resources
We present a mechanistic investigation of bead-based padlock rolling circle amplification (RCA) under molecular crowding conditions, a sensitive and selective DNA detection method we have developed. Several important points to optimize the method were clarified: (i) the increase in the number of RCA products is proportional to the excluded volume of poly(ethylene glycol) (PEG), (ii) PEG facilitates ligation of padlock probe to form circular concatemers and monomers, both of which may act as a template for RCA, and (iii) hybridization of detection probe to the products may be facilitated at higher PEG concentrations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available