Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 412, Issue 1, Pages 93-101Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-019-02209-y
Keywords
Vibrio parahaemolyticus; Polymerase spiral reaction; Isothermal nucleic acid testing; Rapid detection
Funding
- National Natural Science Foundation of China [81502849, 81872668]
- Bethune Medical Scientific Research Fund Project of Jilin University [2018B20]
- Scientific and Technological Research Project of Jilin Province [20170204003SF, 20180101095JC]
- Health science and technology capacity improvement project of Jilin Province [2019Q011]
- Fundamental Research Funds for the Central Universities
Ask authors/readers for more resources
The aim of this study was to develop an effective and specific visual method to rapidly detect and identify Vibrio parahaemolyticus (V. parahaemolyticus) based on the polymerase spiral reaction (PSR). The method utilized only two pairs of primers designed specifically to target the conserved tlh gene sequence of V. parahaemolyticus. Nucleic acid amplification can be achieved under isothermal conditions using DNA polymerase. The reaction could be accomplished in < 40 min with high specificity and sensitivity. The limits of detection of V. parahaemolyticus in purified genomic DNA and pure culture were 300 fg/mu L and 2.4 CFU/mL per reaction, respectively, which were 100-fold more sensitive than with conventional PCR. The model food samples showed consistent specificity and sensitivity to the pure bacterial culture. With these encouraging results, it is expected that the novel, effortless and reliable isothermal nucleic acid testing assay developed in this study has potential to be applied to screening for V. parahaemolyticus in seafood samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available