Journal
ANALYTICA CHIMICA ACTA
Volume 1107, Issue -, Pages 40-47Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.02.006
Keywords
Circulating tumor DNA; Electrochemical biosensor; Nest hybridization chain reaction; Cancer diagnosis
Categories
Funding
- National Natural Science Foundation of China [81802114, 81871735]
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As an ideal biomarker candidate, circulating tumor DNA (ctDNA) plays a vital role in noninvasive diagnosis of cancer. However, most traditional approaches for quantifying ctDNA are cumbersome and expensive. In the present work, a novel electrochemical biosensor based on nest hybridization chain reaction was proposed for the sensitive and specific detection of PIK3CA E545K ctDNA with a simple process. The nest hybridization chain reaction was initiated by the hybridization of two dumbbell-shaped DNA units which were assembled by two classes of well-designed DNA probes respectively, leading to the formation of a complex DNA structure. In the presence of target ctDNA, the amplified hybridization chain reaction products were captured by target ctDNA, resulting in a significant increase of electrochemical signal. Under the optimal conditions, the developed biosensor exhibited good analytical performance for the detection of target ctDNA with the linear range from 5 pM to 0.5 nM and the detection limit of 3 pM. Furthermore, this assay was successfully applied to the detection of ctDNA in spiked-in samples, pleural effusion and serum samples of malignant tumor patients. This simple and costeffective sensing system holds great potentials for ctDNA detection and cancer diagnosis. (C) 2020 Elsevier B.V. All rights reserved.
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