Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 30, Issue 15, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201902463
Keywords
bioscaffold; islet transplantation; oxygen; type 1 diabetes
Categories
Funding
- National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health (NIH) [P30DK116074]
- Akiko Yamzaki and Jerry Yang Faculty Scholar Fund in Pediatric Translational Medicine
- Stanford Maternal and Child Health Research Institute
- Society of Interventional Radiology Foundation Ring Development Grant
- Stanford Nano Shared Facilities (SNSF) [1161726-146-DAARZ]
- National Science Foundation [ECCS-1542152]
- Stanford Neuroscience Microscopy Service grant [NIH NS069375]
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The aim of this article is to develop, characterize, and test a novel 3D bioscaffold matrix that can accommodate pancreatic islets and provide them with a continuous, controlled, and steady source of oxygen to prevent hypoxia-induced damage following transplantation. Hence, a collagen-based cryogel bioscaffold that incorporates calcium peroxide (CPO) into its matrix is made. The optimal concentration of CPO integrated into bioscaffolds is 0.25 wt% and this generates oxygen at 0.21 +/- 0.02 x 10(-3) m day(-1) (day 1), 0.19 +/- 0.01 x 10(-3) m day(-1) (day 6), 0.13 +/- 0.03 x 10(-3) m d(-1) (day 14), and 0.14 +/- 0.02 x 10(-3) m d(-1) (day 21). Accordingly, islets seeded into cryogel-CPO bioscaffolds have a significantly higher viability and function compared to islets seeded into cryogel alone bioscaffolds; these findings are supported by data from quantitative computational modeling. When syngeneic islets are transplanted into the epididymal fat pad (EFP) of diabetic mice, the cryogel-0.25 wt%CPO bioscaffold improves islet function with diabetic animals re-establishing glycemic control. Mice transplanted with cryogel-0.25 wt%CPO bioscaffolds show faster responses to intraperitoneal glucose injections and have a higher level of insulin content in their EFP compared to those transplanted with islets alone (P < 0.05). The novel oxygen-generating bioscaffold (i.e., cryogel-0.25 wt%CPO) therefore provides a biostable and biocompatible 3D microenvironment for islets which can facilitate islet survival and function at extra-hepatic sites of transplantation.
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