Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 12, Issue 8, Pages 9814-9823Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b22601
Keywords
covalent organic frameworks; inherent hydrophilicity; glycopeptides; hydrophilic interaction chromatography; human saliva
Funding
- National Key R&D Program of China [2018YFA0507501]
- National Natural Science Foundation of China [21425518]
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In this work, a magnetic covalent organic framework (COF) with inherent hydrophilicity (denoted mCTpBD) was synthesized through interface deposition of a hydrophilic COF shell on amino group-functionalized magnetite particles via the reaction between a carboxyl group-containing monomer and benzidine. Thanks to the superior hydrophilicity, appropriate porous structure, and easy magnetic separation, the resulting mCTpBD exhibited excellent performance in conveniently enriching glycopeptides from standard samples with a high sensitivity of 0.5 fmol mu L-1 and strong size-exclusion effect of up to 1:1000 (w/w). Furthermore, by using the mCTpBD adsorbent, endogenous glycopeptides in saliva of healthy people and patients with inflammatory bowel disease were successfully enriched and identified by the combined liquid chromatography-mass spectrometry/mass spectrometry technology, which indicates a promising prospective of core shell magnetic composite microspheres with a hydrophilic COF shell in glycoproteomics research.
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