Journal
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12906-016-1360-1
Keywords
HOTAIR; Gastric cancer; Dioscin; lncRNA
Categories
Funding
- Jiangsu provincial clinical medical project [BL2014100]
- Basic clinical research of national Tradition Chinese Medicine project [JDZX2012087]
- Jiangsu provincial health project [BJ14013]
- Changzhou Municipal Health Technology projects [WZ201529]
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Background: As a member of non-coding RNAs family, long non-coding RNAs' functions in cancer needs to be further investigated. It has been indicated that the functions of Hox transcript antisense intergenic RNA (lncRNA: HOTAIR) include reprogramming chromatin organization and promoting tumor metastasis such as breast and colorectal tumor. The aim of this study is to investigate the functions of Hox in gastric cancer. Methods: In the present study, the expression level of HOTAIR was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 20 gastric cancer tissues and 20 normal tissues was included. All clinical data were analyzed retrospectively. The CCK-8 and colony formation assay was used to identify if the knockdown of HOTAIR have an influence on gastric cancer cell lines. Results: Compared with normal tissues, higher expression level of HOTAIR was found in gastric cancer tissues. Dioscin inhibits proliferation of the three gastric cancer cell lines and decrease HOTAIR expression. Conclusions: The expression of HOTAIR is up regulated in gastric cancer and gastric cancer cell lines, dioscin inhibits the proliferation of three gastric cancer cell lines and the anti-tumor effect of dioscin may partly depend on the down regulation of HOTAIR.
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