Journal
BIOPOLYMERS
Volume 104, Issue 5, Pages 552-559Publisher
WILEY
DOI: 10.1002/bip.22695
Keywords
IgY immunoglobulins; fibrinogen binding protein; Staphylococcus aureus; complement C3; peptidyl epitope
Categories
Funding
- National Center for Research and Development [LIDER/08/90/l-1/09/NCBiR/2010]
- Wroclaw University of Technology [S30134/Z0313]
Ask authors/readers for more resources
The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococcus aureus fibrinogen binding protein (Efb) and its peptidyl epitope (spanning residues 127-140). The Efb is an extracellular, adhesion protein which binds both human fibrinogen and complement C3 protein thus contributing to the high infectious potential of this pathogen. The selected epitope of Efb protein is responsible for the interaction with C3. The immunochemical characterization of both anti-Efb and epitope-specific IgY antibodies revealed their similar avidity, titer, and reactivity profile, although some differences in the hen's immune response to administered antigens is discussed. (C) 2015 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available