Microfluidic-CFPA Chip for the Point-of-Care Detection of African Swine Fever Virus with a Median Time to Threshold in about 10 min

The outbreak of African swine fever has brought serious public safety problems to the world, and there is a need for reliable diagnostic tools to prevent and control the epidemic. Here, we successfully established a portable microfluidic-circular fluorescent probe-mediated isothermal nucleic acid amplification (CFPA) system for the rapid, high-throughput, accurate, and sensitive detection of African swine fever virus at point-of-care settings. This detection system has a detection limit of 10 copies/mu L, good stability (C.V. < 5%), and 92.73% sensitivity, and 100% specificity when tested on 220 pig samples collected for the diagnosis of African swine fever virus, with a median time to threshold of 10.8 min. This novel integrated diagnostic tool is urgently needed and has promising applications for the monitoring and control of African swine fever epidemics worldwide and, in particular, the serious outbreak in China.