Journal
BIOPOLYMERS
Volume 103, Issue 6, Pages 321-327Publisher
WILEY
DOI: 10.1002/bip.22615
Keywords
dextran; dextranase; intestinal bacteria; dextran-oligosaccharides; bacteroidetes
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Funding
- National Research Foundation of Korea (NRF) (Ministry of Education, Science and Technology) [2012R1A1B3003708]
- Korea Rural Development Administration (RDA) [PJ90715303]
- National Research Foundation of Korea [2012R1A1B3003708] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The aim of this study was to isolate dextran-hydrolyzing bacteria from the human intestines and to identify their dextranolytic enzymes. For this, dextranase-producing microorganisms were screened from fecal samples by using blue dextran-containing media. Colonies producing a decolorized zone were isolated and they were grouped using RAPD-PCR. 16S rRNA gene sequencing analysis revealed the isolates were Bacteroides (B.) thetaiotaomicron, B. ovatus, B. vulgatus, B. dorei, B. xylanisolvens, B. uniformis, and Veillonella (V.) rogosae. Thin layer chromatography analysis showed that the dextranases exhibit mainly endo-type activity and produce various oligosaccharides including isomaltose and isomaltotriose. Zymogram analysis demonstrated that enzymes localized mainly in the cell membrane fraction and the molecular weight was 50-70 kDa. When cultured in a dextran-containing medium, all strains isolated in this study produced short-chain fatty acids, with butyric acid as the major compound. This is the first study to report that human intestinal B. xylanisolvens, B. dorei, and V. rogosae metabolize dextran utilizing dextranolytic enzymes. (c) 2015 Wiley Periodicals, Inc. Biopolymers 103: 321-327, 2015.
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