Journal
PEERJ
Volume 7, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.7781
Keywords
Phylogenetic analysis (phylogeny); Edwardsiella tarda challenge; Japanese flounder (paralichthys olivaceus); Heat shock protein 70 (hsp 70); Embryo development dynamics
Categories
Funding
- National Key R&D Program of China [2018YFD0900301]
- AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-ES06]
- Taishan Scholar Project Fund of Shandong of China
- National Ten-Thousands Talents Special Support Program
- International Scientific Partnership Program ISPP at King Saud University [0050]
Ask authors/readers for more resources
Flatfish undergo extreme morphological development and settle to a benthic in the adult stage, and are likely to be more susceptible to environmental stress. Heat shock proteins 70 (hsp70) are involved in embryonic development and stress response in metazoan animals. However, the evolutionary history and functions of hsp70 in flatfish are poorly understood. Here, we identified 15 hsp70 genes in the genome of Japanese flounder (Paralichthys olivaceus), a flatfish endemic to northwestern Pacific Ocean. Gene structure and motifs of the Japanese flounder hsp70 were conserved, and there were few structure variants compared to other fish species. We constructed a maximum likelihood tree to understand the evolutionary relationship of the hsp70 genes among surveyed fish. Selection pressure analysis suggested that four genes, hspa4l, hspa9, hspa13, and hyou1, showed signs of positive selection. We then extracted transcriptome data on the Japanese flounder with Edwardsiella tarda to induce stress, and found that hspa9, hspa12b, hspa4l, hspa13, and hyou1 were highly expressed, likely to protect cells from stress. Interestingly, expression patterns of hsp70 genes were divergent in different developmental stages of the Japanese flounder. We found that at least one hsp70 gene was always highly expressed at various stages of embryonic development of the Japanese flounder, thereby indicating that hsp70 genes were constitutively expressed in the Japanese flounder. Our findings provide basic and useful resources to better understand hsp70 genes in flatfish.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available