Journal
MICROMACHINES
Volume 10, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/mi10120837
Keywords
microfluidic valve; competitive enzyme-linked immunosorbent assay (ELISA); pesticide residue
Categories
Funding
- Ministry of Science and Technology, Taiwan [MOST 105-2221-E-002-111, MOST 107-2627-B-002-001]
- [MOST 108-2321-B-002-036]
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Paper-based technologies have been drawing increasing attentions in the biosensor field due to their economical, ecofriendly, and easy-to-fabricate features. In this paper, we present a time-delay valve mechanism to automate a series of procedures for conducting competitive enzyme-linked immunosorbent assay (ELISA) on a paper-based device. The mechanism employs a controllable time-delay valve, which has surfactants to dissolve the hydrophobic barriers, in a fluid pathway. The valves can regulate the liquid and sequentially deliver the sample flow for automating ELISA procedures in microchannels. Competitive ELISA is achieved in a single step once the sample, or small molecule pesticide (e.g., Imidacloprid), is applied onto the paper-based device with a comparable sensitivity to plate-based competitive ELISA. The results further demonstrate the appositeness of using paper-based devices with the valve designs for on-the-go ELISA detection in agriculture and biomedical applications.
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