Journal
MICROMACHINES
Volume 10, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/mi10110783
Keywords
enzyme immobilization; continuous; biocatalysis; imine reductase; microreactor
Categories
Funding
- Helmholtz program Bio-Interfaces in Technology and Medicine
- Fonds der Chemischen Industrie (FCI)
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All-enzyme hydrogels are biocatalytic materials, with which various enzymes can be immobilized in microreactors in a simple, mild, and efficient manner to be used for continuous flow processes. Here we present the construction and application of a cofactor regenerating hydrogel based on the imine reductase GF3546 from Streptomyces sp. combined with the cofactor regenerating glucose-1-dehydrogenase from Bacillus subtilis. The resulting hydrogel materials were characterized in terms of binding kinetics and viscoelastic properties. The materials were formed by rapid covalent crosslinking in less than 5 min, and they showed a typical mesh size of 67 +/- 2 nm. The gels were applied for continuous flow biocatalysis. In a microfluidic reactor setup, the hydrogels showed excellent conversions of imines to amines for up to 40 h in continuous flow mode. Variation of flow rates led to a process where the gels showed a maximum space-time-yield of 150 g.(L.day)(-1) at 100 mu L/min.
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