Journal
TOXINS
Volume 11, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/toxins11100585
Keywords
venom peptides; FMRF-amide; insect neurotoxin; protons; pH regulation; acid-sensing ion channels; acid-gated currents
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Funding
- Vicerrectoria de Investigacion y Estudios de Posgrado (VIEP-BUAP)
- Consejo Nacional de Ciencia y Tecnologia de Mexico (CONACyT), Fronteras de la Ciencia [1544]
- CONACyT fellowship [669573]
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In this work, we evaluate the effect of two peptides Sa12b (EDVDHVFLRF) and Sh5b (DVDHVFLRF-NH2) on Acid-Sensing Ion Channels (ASIC). These peptides were purified from the venom of solitary wasps Sphex argentatus argentatus and Isodontia harmandi, respectively. Voltage clamp recordings of ASIC currents were performed in whole cell configuration in primary culture of dorsal root ganglion (DRG) neurons from (P7-P10) CII Long-Evans rats. The peptides were applied by preincubation for 25 s (20 s in pH 7.4 solution and 5 s in pH 6.1 solution) or by co-application (5 s in pH 6.1 solution). Sa12b inhibits ASIC current with an IC50 of 81 nM, in a concentration-dependent manner when preincubation application was used. While Sh5b did not show consistent results having both excitatory and inhibitory effects on the maximum ASIC currents, its complex effect suggests that it presents a selective action on some ASIC subunits. Despite the similarity in their sequences, the action of these peptides differs significantly. Sa12b is the first discovered wasp peptide with a significant ASIC inhibitory effect.
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