Journal
OPEN BIOLOGY
Volume 9, Issue 10, Pages -Publisher
ROYAL SOC
DOI: 10.1098/rsob.190140
Keywords
polyunsaturated fatty acids (PUFAs); n-3; 6 PUFAs; fatty acid desaturase (Fad); Fad2/3; transgenic mice
Categories
Funding
- Genetically Modified Organisms Breeding Major Projects [2016ZX08007-002]
- State Key Laboratory of R2BGL
- Inner Mongolia Autonomous Region Basic Research Project
- Inner Mongolia Science and Technology Innovation Guide Project [KCBJ2018002]
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Linoleic acid (18 : 2, n-6) and alpha-linolenic acid (18 : 3, n-3) are polyunsaturated fatty acids (PUFAs), which are essential for mammalian health, development and growth. However, the majority of mammals, including humans, are incapable of synthesizing n-6 and n-3 PUFAs. Mammals must obtain n-6 and n-3 PUFAs from their diet. Fatty acid desaturase (Fad) plays a critical role in plant PUFA biosynthesis. Therefore, we generated plant-derived Fad3 single and Fad2-Fad3 double transgenic mice. Compared with wild-type mice, we found that PUFA levels were greatly increased in the single and double transgenic mice by measuring PUFA levels. Moreover, the concentration of n-6 and n-3 PUFAs in the Fad2-Fad3 double transgenic mice were greater than in the Fad3 single transgenic mice. These results demonstrate that the plant-derived Fad2 and Fad3 genes can be expressed in mammals. To clarify the mechanism for Fad2 and Fad3 genes in transgenic mice, we measured the PUFAs synthesis-related genes. Compared with wild-type mice, these Fad transgenic mice have their own n-3 and n-6 PUFAs biosynthetic pathways. Thus, we have established a simple and efficient method for in vivo synthesis of PUFAs.
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