Journal
ADVANCED HEALTHCARE MATERIALS
Volume 8, Issue 22, Pages -Publisher
WILEY
DOI: 10.1002/adhm.201901103
Keywords
bilayer hydrogels; hydroxyapatite mineralization; mussel-inspired; osteochondral defect repair; tough hydrogels
Funding
- National Key Research and Development Program of China [2016YFB0700802]
- R&D Program in Key Areas of Guangdong [2019B010941002]
- NSFC [81671824, 51773119, 31700841]
- Fundamental Research Funds for the Central Universities [2682019JQ03]
- Science Technology Innovation Commission of Shenzhen Municipality [JCYJ 20170817171930009, GJHZ20180416164817264]
- Natural Science Foundation of Tianjin [18JCYBJC19500]
- Independent Innovation Fund of Tianjin University [2018XGP-0030]
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Repairing osteochondral defects is a considerable challenge because it involves the breakdown of articular cartilage and underlying bone. Traditional hydrogels with a homogenized single-layer structure cannot fully restore the function of osteochondral cartilage tissue. In this study, a mussel-inspired hydrogel with a bilayer structure is developed to repair osteochondral defects. The hydrogel is synthesized by simultaneously polymerizing two layers using a one-pot method. The resulting upper and lower gelatin methacryloyl-polydopamine hydrogel layers are used as cartilage and subchondral bone repair layers, respectively. Polydopamine-induced hydroxyapatite in situ mineralization takes place in the lower layer to mimic the structure of subchondral bone. The bilayer hydrogel exhibits good mechanical properties for the synergistic effect of covalent and noncovalent bonds, as well as nanoreinforcement of mineralized hydroxyapatite. To improve the tissue-inducibility of hydrogels, transforming growth factor beta(3) is immobilized in the upper layer to induce cartilage regeneration, while bone morphogenetic protein 2 is immobilized in the lower layer to induce bone regeneration. Bone and cartilage repair performance of the hydrogel is examined by implantation into a full-thickness cartilage defect of a rabbit knee joint. The bilayer-structure hydrogel promotes regeneration of osteochondral tissue, thus providing a new option for repair of osteochondral defects.
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