Journal
ACS SYNTHETIC BIOLOGY
Volume 8, Issue 12, Pages 2620-2628Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.9b00166
Keywords
synthetic biology; biosensors; microbiome engineering; E. coli Nissle 1917; Caenorhabditis elegans
Categories
Funding
- BBSRC LIDo Doctoral Training Partnership
- MRC
- BBSRC
- Microsoft Research Scholarship
- EPSRC
- Wellcome Trust/Royal Society [102531/Z/13/A]
- MRC [MC-A654-5QC80]
- Wellcome Trust [097319/Z/11/Z]
- European Research Council (ERC) under the European Union [770835]
- Wellcome Trust [097319/Z/11/Z] Funding Source: Wellcome Trust
- BBSRC [1763910] Funding Source: UKRI
- MRC [MC_UP_1605/6] Funding Source: UKRI
- European Research Council (ERC) [770835] Funding Source: European Research Council (ERC)
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Caenorhabditis elegans has become a key model organism within biology. In particular, the transparent gut, rapid growing time, and ability to create a defined gut microbiota make it an ideal candidate organism for understanding and engineering the host microbiota. Here we present the development of an experimental model that can be used to characterize whole-cell bacterial biosensors in vivo. A dual-plasmid sensor system responding to isopropyl beta-D-1-thiogalactopyranoside was developed and fully characterized in vitro. Subsequently, we show that the sensor was capable of detecting and reporting on changes in the intestinal environment of C. elegans after introducing an exogenous inducer into the environment. The protocols presented here may be used to aid the rational design of engineered bacterial circuits, primarily for diagnostic applications. In addition, the model system may serve to reduce the use of current animal models and aid in the exploration of complex questions within general nematode and host-microbe biology.
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