Journal
SCIENTIFIC REPORTS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-019-51319-1
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Funding
- Association of Bioeconomy Research (FBF e.V.)
- Rentenbank - Germany's development agency for agribusiness [AMIKOS 823 600]
- Deutsche Forschungsgemeinschaft
- University of Veterinary Medicine Hannover, Foundation
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The role of antibiotics (AB) in semen extenders as a potential contribution to the global antimicrobial resistance threat is emerging. Here, we establish an AB-free hypothermic preservation strategy for boar semen and investigate its impact on sperm function, microbial load and fertility after artificial insemination (AI). Spermatozoa (12 boars) preserved in AB-free AndroStar Premium extender at 5 degrees C maintained high motility, membrane integrity, and a low DNA-fragmentation index throughout 72 h storage and results did not significantly differ from controls stored at 17 degrees C in extender containing AB (p = 0.072). Likewise, kinetic response of spermatoza to the capacitation stimulus bicarbonate during 180 min incubation in Tyrode's medium did not differ from 17 degrees C-controls. In a competitive sperm oviduct binding assay, binding indices did not differ between semen stored for 72 h AB-free at 5 degrees C and 17 degrees C-controls (n = 6 boars). Bacterial load < 10(3) CFU/ml after 72 h was measured in 88.9% of samples stored at 5 degrees CAB-free compared to 97.2% in 17 degrees C-controls (n = 36 semen pools, 23 boars). Fertility traits of 817 females did not differ significantly between the two semen groups (p > 0.05). In conclusion, a hypothermic semen preservation strategy is presented which offers antibiotic-free storage of boar semen doses.
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