Journal
NUTRIENTS
Volume 11, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/nu11112564
Keywords
microbiota; 16S rRNA sequencing; vitamin D deficit
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Funding
- Mineco [SAF2016-77222-R, SAF2017-8489-R]
- European Union (Fondo Europeo de Desarrollo Regional FEDER)
- Fundacion Contra la Hipertension Pulmonar (Empathy grant)
- Mineco (FPU grant)
- Universidad Complutense de Madrid
- Mobility grant from CIBERES
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Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on alpha- diversity but no effect on beta-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.
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