Journal
MATERIALS
Volume 12, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/ma12233932
Keywords
bioactive surfaces; biomaterials; hybrid organometallic polymers; laser two-photon polymerisation; tissue engineering
Categories
Funding
- INFOBALT Lithuania
- World Federation of Scientists
- European Commission via the Marie Sklodowska-Curie research fellowship programme AngioMatTrain [317304]
- Lancaster University Faculty of Science and Technology Early Career Internal Grant
- Royal Society Research Grant [RG160449]
- EPSRC First Grant [EP/R003823/1]
- Research Council of Lithuania [SEN-13/2015]
- EPSRC [EP/R003823/1] Funding Source: UKRI
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Hybrid organometallic polymers are a class of functional materials which can be used to produce structures with sub-micron features via laser two-photon polymerisation. Previous studies demonstrated the relative biocompatibility of Al and Zr containing hybrid organometallic polymers in vitro. However, a deeper understanding of their effects on intracellular processes is needed if a tissue engineering strategy based on these materials is to be envisioned. Herein, primary rat myogenic cells were cultured on spin-coated Al and Zr containing polymer surfaces to investigate how each material affects the viability, adhesion strength, adhesion-associated protein expression, rate of cellular metabolism and collagen secretion. We found that the investigated surfaces supported cellular growth to full confluency. A subsequent MTT assay showed that glass and Zr surfaces led to higher rates of metabolism than did the Al surfaces. A viability assay revealed that all surfaces supported comparable levels of cell viability. Cellular adhesion strength assessment showed an insignificantly stronger relative adhesion after 4 h of culture than after 24 h. The largest amount of collagen was secreted by cells grown on the Al-containing surface. In conclusion, the materials were found to be biocompatible in vitro and have potential for bioengineering applications.
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