Journal
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
Volume 19, Issue 2, Pages 139-147Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1534734619880055
Keywords
foot ulcers; adipose-derived stem cells; collagen scaffold; wound healing
Categories
Funding
- National Natural Science Foundation of China [81671922]
- Natural Science Foundation of Jiangsu Province [BK20170124]
- Project of Nanjing Science and Technology Development [YKK16077]
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This study aimed at evaluating the effects of collagen scaffold with Bcl-2-modified adipose-derived stem cells (ADSCs) on wound repair in streptozotocin-induced diabetic mice. A round full thickness skin defect with a diameter of 7 mm was made in the mice model. The experimental mice were divided into 4 groups (n = 12 each): group A (control group), group B (scaffold group), group C (ADSCs-scaffold group), and group D (Bcl-2-ADSCs-scaffold group). On days 3, 7, 10, and 14 after surgery, characteristics of wound healing was observed, and wound tissues were sampled for histology characteristics via hematoxylin-eosin staining and immunohistochemical staining. Compared with other groups, the wound healing rate was significantly higher in group D a week after operation (P < .05). On the seventh day postoperation, group D exhibited higher blood vessel in the wounds granulation tissue than other groups according to results of hematoxylin-eosin staining and immunohistochemistry. In conclusion, these findings demonstrated that collagen scaffold with Bcl-2 modified ADSCs may effectively improve the wound healing process in diabetic mice.
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